Also Known As:129S1 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
Why are C57BL 6 mice used?
C57BL/6 mice is an inbred mouse strain originally developed for the study of antitumor activity and immunology, and is increasingly being used as an basic background strain. It is known that cell-mediated immunity and NK cell activity are of relatively high importance in determining immunological characteristics.
How do you write a genotype for a mouse?
Thus, we designate our mouse genotypes “-/-” for homozygous mutants, “+/-” for heterozygotes, and “+/+” for wildtypes.
What is the difference between C57BL 6 and BALB C?
BALB/c mice tend to produce a stronger humoral response than C57BL/6 mice. These strains have different gene sequences at the H2 site of the major histocompatibility (MHC)class I gene locus – BALB/c mice is H2d, while C57BL/6 is H2b. The difference in gene sequence of these two strains falls on the H2 locus.
Are C57BL 6 mice aggressive?
Although some consider C57BL/6 mice to be aggressive, in general they are not when compared to other mouse strains. However, they may appear to be more aggressive because they are the most commonly used mouse strain in research and thus there are many of them to observe.
How do you write mutants?
The full descriptive names of the wild-type (ALPHABETICA) and mutant (alphabetica) alleles should be written in the same manner. Protein products of genes should be written in capital letters without italics (ABC). Phenotypes may be designated by the gene symbol (no italics) with the first letter capitalized.
Can you cross two Cre lines?
Assuming you are crossing two cre-driver lines, it is possible, but the number of mice required may become quite substantial depending on your intended outcome. If either of these lines expresses in the testes or ovaries, it will generate a germline mutation for the loxP flanked gene of interest.
Do BALB c mice have an immune system?
The structural and functional parameters of the immune system in BALB/c and C57Bl/6 mice differ under physiological conditions. However, IL-12 production in BALB/c mice was lower than in C57Bl/6 mice. C57Bl/6 mice were characterized by higher cytostatic activity of splenic NK cells.
Why are BALB c mice used?
BALB/c mice are useful for research into both cancer and immunology. Most substrains have a “long reproductive life-span”, are noted for displaying high levels of anxiety and for being relatively resistant to diet-induced atherosclerosis, making them a useful model for cardiovascular research.
How do you write mammalian genes?
Mice and rats: Gene symbols are italicized, with only the first letter in upper-case (e.g., Gfap). Protein symbols are not italicized, and all letters are in upper-case (e.g., GFAP). Fish: In contrast to the general rule, full gene names are italicized (e.g., brass).
Why is the nomenclature for mice 129?
Because of the importance of 129 mice in creating “knockout” and other targeted mutant mice, the International Committee on Standardized Genetic Nomenclature for Mice introduced a nomenclature to distinguish different 129 parental lines and related 129 strains.
What is the history of the 129s2 mouse strain?
Charles River France acquired this strain in August 1996. The strain was renamed 129S2 in 1999 after the revision of the international nomenclature. Note: We also offer the 129-Elite Mouse with a VAF/Elite ® health profile (bred in US) and the 129-SOPF Mouse with a SOPF health profile (bred in France).
Can 129 SV cells be mated with 129 SV mice?
The ES cell lines derived from 129/Sv mice are reputed to have a high incidence of germline transmission and are easily maintained in culture. Chimeric, knockout-positive mice produced from these cells may be mated to 129/SvEv inbred mice to increase and preserve the inbred background of the knockout line.
Where did 129129 mice come from?
129 Mice | Charles River Laboratories. Developed by Dr. L.C. Stevens from The Jackson Laboratory. During the 1970s, Dr. Stevens introduced this line to the Pasteur Institute of Paris in the laboratory of Dr. J.L. Guenet. Charles River France acquired this strain in August 1996.
